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KMID : 0368420080510050359
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Journal of Plant Biology
2008 Volume.51 No. 5 p.359 ~ p.365
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A sterol biosynthetic geneAtCYP51A2 promoter for constitutive and ectopic expression of a transgene in plants
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Lee Hyoung-Seok
Oh Hyun-Jeong
Ahn Hyo-Min
Oh Chang-Jae
Jeong Jin-Ho
Jeon Gyeong-Lyong
An Chung-Sun
Choi Sang-Bong
Kim Ho-Bang
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Abstract
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Arabidopsis CYP51A2 (AtCYP51A2) mediates the sterol 14α-demethylation step inde novo sterol biosynthesis, and is constitutively and highly expressed in all plant tissues (Kim et al., 2005). We exploited the molecular features of its expression and the fundamental role of sterol biosynthesis in cells to develop a plant-derived promoter. Our GUS expression analysis between transgenicArabidopsis lines forAtCYP51A2::GUS and35S::GUS revealed that activity of theAtCYP51A2 promoter was comparable to that of the35S promoter, based on enzymatic activities and protein levels. TheAtCYP51A2 promoter was also constitutively active in transgenic tobacco, indicating that 5′ regulatory elements could be conserved amongCYP51 promoters in dicot plants. A homologue ofAtCYP51A2 was identified from rape seed, a crop species closely related toArabidopsis. Its constitutive tissue expression pattern implies that the application of thisAtCYP51A2 promoter is possible for that species. Based on these results, we present a new binary vector system with the plant-derivedAtCYP51A2 promoter, which is able to constitutively and ectopically drive a transgene in various dicotyledonous plants.
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KEYWORD
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Arabidopsis CYP51A2 promoter, β-glucuronidase, binary vector, CaMV 35S promoter, constitutive overexpression, sterol biosynthesis
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